Catalog NumberHG6004246
CAS Number6004-24-6
CategoryCationic Surfactants
Use as antistatic agent.
Use as emulsifying agent, dispersing agent.
Use as flocculant.
Use as deodorant.
Utilizing Cetylpyridinium Chloride Monohydrate in the Development of a Novel Antimicrobial Complex
Dubovoy, Viktor, et al. ACS omega 5.18 (2020): 10359-10365.
This work described how cetylpyridinium chloride monohydrate (CPC·H2O) functioned as the cationic surfactant/raw material in the preparation of a cetylpyridinium-zinc solid (cetylpyridinium tetrachlorozincate, (CP)2ZnCl4), and summarized the resulting material's structure, antimicrobial performance, and incorporation into mesoporous supports as reported.
Synthetic Procedure: The synthesis was initiated using commercial Cetylpyridinium Chloride Monohydrate and anhydrous Zinc Chloride. A fresh 25% by weight aqueous solution of CPC was prepared. A separate 75% by weight solution of zinc chloride was then added dropwise to the CPC solution under constant magnetic stirring. This reaction produced a solid precipitate, which was isolated via vacuum filtration and dried. The crude product was subsequently purified using an acetone extraction process, and a single crystal suitable for analysis was obtained through recrystallization from acetone.
Structure and Antimicrobial Performance
· Single-crystal X-ray diffraction determined the product stoichiometry as C42H76Cl4N2Zn, corresponding to two cetylpyridinium cations per [ZnCl4]2- anionic unit ((CP)2ZnCl4). Crystallography at 100 K and 298 K revealed a zig-zag arrangement with alternating alkyl chains and zinc-containing units in the solid state.
· MICs of (CP)2ZnCl4 were reported as: Salmonella enterica: 60 μg/mL, Staphylococcus aureus: 6 μg/mL, Streptococcus mutans: 6 μg/mL. The MICs of the zinc-containing solid were comparable to those of pure CPC, despite the material's composition in which approximately 16% of the bactericidal CPC is replaced by bacteriostatic ZnCl2 in the combined structure.
Cetylpyridinium Chloride: Efficacy of CPC-Containing Mouthwash Against SARS-CoV-2
D'Amico, Filippo, et al. Molecular Oral Microbiology 38.3 (2023): 171-180.
This study summarized randomized controlled-trial evidence on the ability of cetylpyridinium chloride (CPC) mouthwash to reduce salivary SARS-CoV-2 viral load and discuss practical implications for CPC as an oral antiviral intervention.
Key Findings
· In six randomized trials, CPC-containing mouthwashes were reported to be effective in vivo in reducing salivary SARS-CoV-2 viral load versus placebo and versus other mouthwash active ingredients in the majority of studies.
· In one trial, no statistically significant differences versus placebo were reported for any of the mouthwashes tested (though favorable trends were noted for CPC and povidone-iodine), while in five trials, CPC was reported to be more effective at reducing salivary viral load than other common mouthwash actives (hydrogen peroxide, chlorhexidine, povidone-iodine); one of these reported a statistically significant reduction for CPC.
· Observed duration of reduced salivary viral load extended up to ~6 hours after rinsing with 10-20 mL of 0.05%-0.1% CPC for 30 s to 1 min in the clinical studies.
· In vitro studies showed that 0.07% CPC can inactivate ≥99.9% of SARS-CoV-2 within 30 s to 2 min in tissue culture, including in the presence of sterilized saliva. Proposed antiviral mechanisms from the literature include disruption of the viral lipid envelope and interference with spike-protein - ACE2 interactions.
Developing a Dual-Action Antibacterial Material Using Cetylpyridinium Chloride
Yapar, Saadet, et al. Applied Clay Science 150 (2017): 16-22.
Cetylpyridinium chloride (CPC) can modify montmorillonite (Mt) clay and be the cationic anchor for subsequent adsorption of the anionic surfactant sodium lauroyl sarcosinate (SR) to provide a composite (Mt-CP-SR) that has antibacterial activity and may have potential for personal-care applications.
Preparation: The synthesis was conducted in a two-step process. First, an aqueous dispersion of montmorillonite (Mt) was modified with Cetylpyridinium Chloride at specific ratios relative to the clay's cation exchange capacity (CEC). The mixture was agitated for 24 hours. In the second step, sodium lauroyl sarcosinate (SR) was added to the CP-modified clay (Mt-CP) dispersion in varying proportions and shaken for another 24 hours. The final solid product (Mt-CP-SR) was isolated via filtration and dried.
Key Results:
· Approximately 140 mg SR per gram of Mt could be loaded onto Mt pre-intercalated with ~170 mg CPC per gram. SR desorption into water was gradual, indicating strong interactions between SR and the CPC-modified montmorillonite surface.
· In antimicrobial testing, sodium lauroyl sarcosinate (SR) solutions showed activity against all three organisms with MICs of 75 mg/mL for E. coli, 37.5 mg/mL for S. aureus, and 300 mg/mL for P. aeruginosa, and an MBC of 300 mg/mL for E. coli and S. aureus; equimolar CP+SR mixtures were effective against S. aureus but ineffective against P. aeruginosa, while the final Mt-CP-SR composite exhibited antibacterial activity against both S. aureus and E. coli under the reported test conditions.