Mbandi, Evelyne, et al. Journal of food protection 70.1 (2007): 58-64.
Listeria monocytogenes is a major concern for RTE meats, but the underlying proteomic effects of the organic acid salts are unknown. This case study assesses the individual and combined antimicrobial effects of sodium lactate (NaLA) and sodium diacetate (SD) to reduce L. monocytogenes expression profiles.
Key Findings:
· Differential Protein Impact: SDA treatment caused the most profound proteomic shifts (124 unmatched proteins, 20 upregulated, 90 novel). NaLA treatment showed significant but less extensive changes (53 unmatched, 5 upregulated, 45 novel). NaLA+SDA combination produced strongest suppression (41 downregulated proteins vs. ~30 for single salts)
· Critical Protein Alterations: Upregulated oxidoreductase, lipoprotein (stress response); Downregulated DNA-binding protein, alpha amylase, SecA proteins (metabolism/secretion disruption).
· Synergistic Advantage: Combination leveraged complementary mechanisms, including SDA induced novel stress-response proteins, NaLA enhanced suppression of essential functions, and Joint treatment maximized virulence pathway disruption.
· Sodium lactate's synergy with sodium diacetate creates superior antimicrobial action through complementary proteomic impacts, validating NaLA+SDA blends (2.5% NaLA + 0.2% SD) as optimal for RTE meat protection.