Al-Obaidy, Saba SM, et al. Pharmaceutics 13.9 (2021): 1389.
Chlorhexidine (CHX) is widely used as an endodontic irrigant, although it can encounter efficacy problems against resistant microbes. In this study, we evaluated the effect of the addition of 0.2% cetylpyridinium chloride (CPC) cationic surfactant to 2% CHX as an enhancer of the antimicrobial properties of CHX.
Methodology: The test product, a 2% CHX+0.2% CPC combination irrigant formulation, was assessed for in vitro antimicrobial activity and compared to a negative control (2% CHX alone) and 100% MTAD (positive control; a commercial irrigant with known antibacterial efficacy). The samples were tested against E. faecalis, S. aureus, and C. albicans bacteria and fungi.
Key Results:
· Against E. faecalis: All irrigants-CHX, CHX+CPC, and MTAD-showed similar levels of antimicrobial activity. The CPC addition did not significantly alter CHX efficacy.
· Against S. aureus: 2% CHX was significantly more effective than MTAD. The CHX+CPC combination showed equivalent efficacy to CHX alone, suggesting CPC does not diminish CHX's antibacterial action.
· Against C. albicans: Both CHX and CHX+CPC were markedly more effective than MTAD. Notably, CPC improved the formulation's antifungal potency without compromising its stability or performance.
Karpiński, T. M., et al. Eur Rev Med Pharmacol Sci 19.7 (2015): 1321-1326.