Solana, Carmen, et al. Journal of endodontics 43.12 (2017): 2093-2096.
Removing biofilm from root canals is a major challenge for root canal disinfection, especially with respect to resistant microorganisms like Enterococcus faecalis. This case report shows how alkaline tetrasodium EDTA (EDTANa4) acts synergistically with sodium hypochlorite (NaOCl) to eliminate biofilm without reducing the antibacterial effect.
Test Solutions: Control: 0.9% saline; Individual agents: 2.5% NaOCl, 10% EDTANa4, 20% EDTNa4; Combination therapies: a) NaOCl/EDTANa4 blends (2.5%/5% and 2.5%/10%), b) NaOCl/EDTANa4/0.2% cetrimide (CTR) formulations.
Key Findings:
· Synergistic Performance: NaOCl/EDTANa4 Blends Achieved complete bacterial eradication (0 CFU), matching 2.5% NaOCl alone. EDTNa4 Alone Showed lowest efficacy. CTR-Enhanced Formulations (2.5% NaOCl/10% EDTANa4/0.2% CTR) demonstrated equivalent biofilm elimination to NaOCl monotherapy.
· Critical Advantage: EDTANa4 (Alkaline) did not reduce the antimicrobial effect of NaOCl in combination.
· Alkaline tetrasodium EDTA chelating agent and NaOCl used together would significantly reduce the time of treatment and avoid any interaction between the agents which could affect the potency of NaOCl. Thus, NaOCl/alkaline EDTANa4 can be used as a substitute for the single irrigation solutions.
Jang, Youngjin, et al. Nanomaterials 8.4 (2018): 252.